Nipah virus conforms to the rule of six in a minigenome replication assay
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Nipah virus conforms to the rule of six in a minigenome replication assay.
To study the replication of Nipah virus (NiV), a minigenome replication assay that does not require the use of infectious virus was developed. The minigenome was constructed to encode a NiV vRNA analogue containing the gene for chloramphenicol acetyltransferase (CAT) under the control of putative NiV transcription motifs and flanked by the NiV genomic termini. CAT protein was detected only when...
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Nipah virus (NiV) is a recently emergent, highly pathogenic, zoonotic paramyxovirus of the genus Henipavirus. Like the phosphoprotein (P) gene of other paramyxoviruses, the P gene of NiV is predicted to encode three additional proteins, C, V and W. When the C, V and W proteins of NiV were tested for their ability to inhibit expression of the chloramphenicol acetyltransferase (CAT) reporter gene...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2004
ISSN: 0022-1317,1465-2099
DOI: 10.1099/vir.0.19685-0